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Responsiveness of experimental surgical-wound infections to topical chemotherapy
Abstract:
Topical agents freshly formulated in a cream base vehicle as well as commercial topical preparations were used to evaluate in mice the responsiveness of experimental surgical wounds infected with Staphylococcus aureus or Pseudomonas aeruginosa to chemotherapy. The responsiveness of the infections to therapy or the efficacy of a topical agent was assessed primarily by means of wound counts of the infecting organism before and after the employment of an immediate (prophylactic) or delayed (therapeutic) treatment regimen. From tests of several concentrations of an agent formulated in the vehicle, a median effective dose could be determined. In the case of the lethal P. aeruginosa infection, a median protective dose could be determined. Both infections were found to be quite susceptible to treatment with those topical agents that demonstrated good activity in vitro against the test organisms. The results of the investigation indicated that the model infections were suitable for the screening of potential topical agents in vivo.
Insights
This study evaluated topical agents for wound infections in mice caused by Staphylococcus aureus and Pseudomonas aeruginosa. Effective chemotherapy was demonstrated for both bacterial infections, validating the mouse model for screening new topical treatments.
Area of Science:
- Microbiology
- Pharmacology
- Infectious Diseases
Background:
- Topical antimicrobial agents are crucial for managing wound infections.
- Experimental models are needed to assess the efficacy of novel topical treatments.
- Staphylococcus aureus and Pseudomonas aeruginosa are common pathogens in surgical wound infections.
Purpose of the Study:
- To evaluate the efficacy of topical antimicrobial agents against experimental wound infections in mice.
- To assess the responsiveness of Staphylococcus aureus and Pseudomonas aeruginosa infections to chemotherapy.
- To determine the suitability of this mouse model for screening potential topical agents in vivo.
Main Methods:
- Experimental surgical wound infections were established in mice using Staphylococcus aureus or Pseudomonas aeruginosa.
- Topical agents, freshly formulated or commercial, were administered prophylactically or therapeutically.
- Infection burden was quantified by bacterial wound counts before and after treatment.
- Median effective dose (MED) and median protective dose (MPD) were determined for tested agents.
Main Results:
- Both Staphylococcus aureus and Pseudomonas aeruginosa wound infections showed susceptibility to topical agents with demonstrated in vitro activity.
- The mouse model allowed for the determination of median effective doses for therapeutic agents.
- For lethal Pseudomonas aeruginosa infections, a median protective dose was successfully determined.
Conclusions:
- The tested topical agents were effective in treating experimental wound infections in mice.
- The developed mouse model is suitable for the in vivo screening and evaluation of new topical antimicrobial agents.
- This research provides a foundation for further development of topical chemotherapy for wound infections.