Related Experiment Video

Updated: Aug 14, 2026

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
15:43

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes

Published on: January 7, 2013

[Disorders of the processes of protein biosynthesis and a decrease in the content of cytochromes P-450 and b5 in the

Kh Ia Karimov1, R N Akhmedov, F Kh Inoiatova

  • 1Second Tashkent State Medical Institute, Uzbekistan.

Ukrainskii Biokhimicheskii Zhurnal (1978)
|December 16, 1998
PubMed

Insights

Acute pyelonephritis significantly reduces key kidney proteins, cytochrome P-450 and cytochrome b5. This decrease correlates with disease severity, potentially due to toxic products and increased catabolism in the injured kidney.

Area of Science:

  • Biochemistry
  • Nephrology
  • Pathology

Context:

  • Acute unilateral pyelonephritis impacts kidney function.
  • Microsomal fractions are crucial for metabolic processes within the kidney.
  • Cytochrome P-450 and cytochrome b5 are key microsomal proteins involved in detoxification and metabolism.

Purpose:

  • To investigate the changes in microsomal protein levels, specifically cytochrome P-450 and cytochrome b5, during acute unilateral pyelonephritis.
  • To determine the correlation between the reduction in these proteins and the severity of the pyelonephritis.
  • To explore potential causes for the observed decrease in protein levels.

Summary:

  • Acute unilateral pyelonephritis leads to a notable decrease in cytochrome P-450 and cytochrome b5 content within the kidney's microsomal fraction.
  • The extent of this reduction is directly proportional to the severity and developmental stage of the pyelonephritis.
  • Toxic products from altered metabolism, increased catabolism, and endogenous intoxication are implicated as primary contributors to the diminished protein levels and inhibited protein biosynthesis.

Impact:

  • Highlights the biochemical alterations in the kidney during pyelonephritis.
  • Suggests a link between protein biosynthesis inhibition and disease progression.
  • Underscores the need for further research into the multifactorial causes of microsomal hemoprotein reduction in damaged kidneys.

More Related Videos

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells09:40

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells

Published on: June 20, 2018

Modeling Hypoxia/Reoxygenation Injury in Proximal Tubular Epithelial Cells06:23

Modeling Hypoxia/Reoxygenation Injury in Proximal Tubular Epithelial Cells

Published on: November 21, 2025

Related Concept Videos

Overview of Protein Metabolism01:21

Overview of Protein Metabolism

Proteins are broken down into amino acids during digestion. Unlike fats and carbohydrates, which are stored for later use, proteins are not. Instead, amino acids are either used to produce ATP through oxidation or contribute to the creation of new proteins for the growth and repair of the body. Any surplus amino acids from the diet are converted into glucose or triglycerides rather than excreted.
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...
Nephrons01:10

Nephrons

The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma happens...
Acute Pyelonephritis I: Introduction01:27

Acute Pyelonephritis I: Introduction

Pyelonephritis is a bacterial infection that primarily affects the renal parenchyma and collecting system, including the renal pelvis, tubules, and interstitial tissue of one or both kidneys. It can be classified as either acute—a sudden, severe infection—or chronic, which refers to long-term or recurrent kidney infections.The primary cause of acute pyelonephritis (APN) is bacterial infection, with Escherichia coli accounting for approximately 70-80% of cases. Other bacteria, such as Proteus,...
Acute Pyelonephritis II: Diagnostic Studies and Management01:28

Acute Pyelonephritis II: Diagnostic Studies and Management

Introduction:For diagnosing acute pyelonephritis, a comprehensive patient history is collected to identify symptoms such as dysuria, frequent or urgent urination, flank pain, or costovertebral angle (CVA) tenderness that may suggest a kidney infection.Physical ExaminationDuring the physical examination, CVA tenderness is assessed. This involves gentle percussion over the costovertebral angle, where tenderness often indicates a kidney infection.Diagnostic TestsUrinalysis: Used to identify white...
Acute Kidney Injury II: Pathophysiology01:29

Acute Kidney Injury II: Pathophysiology

Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
Acute Kidney Injury IV: Diagnostic Studies and Prevention01:30

Acute Kidney Injury IV: Diagnostic Studies and Prevention

Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...