Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transformed but not normal hepatocytes express UCP2

M V Carretero1, L Torres, U Latasa

  • 1Departamento de Medicina Interna, Facultad de Medicina, Universidad de Navarra, Pamplona, Spain.

FEBS Letters
|December 16, 1998
PubMed
Summary

Uncoupling protein 2 (UCP2) is expressed in some liver cancer cells but not in normal adult liver cells. Serum factors and hypomethylation may regulate UCP2 expression in hepatocarcinoma.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolomic-based aging clocks.

npj metabolic health and disease·2025
Same author

Ubiquitous calpains show differential dynamics and function during adipocyte differentiation.

Archives of biochemistry and biophysics·2025
Same author

Cleavage and activation of LIM kinase 1 as a novel mechanism for calpain 2-mediated regulation of nuclear dynamics.

Scientific reports·2021
Same author

S-adenosyl-L-methionine (SAMe) halts the autoimmune response in patients with primary biliary cholangitis (PBC) via antioxidant and S-glutathionylation processes in cholangiocytes.

Biochimica et biophysica acta. Molecular basis of disease·2020
Same author

A fast method for the quantitation of key metabolites of the methionine pathway in liver tissue by high-resolution mass spectrometry and hydrophilic interaction ultra-performance liquid chromatography.

Analytical and bioanalytical chemistry·2013
Same author

Spanish human proteome project: dissection of chromosome 16.

Journal of proteome research·2012

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Hepatology

Background:

  • Uncoupling protein 2 (UCP2) expression in the liver is primarily localized to non-parenchymal cells.
  • UCP2 is absent in normal adult hepatocytes but present in some hepatocarcinoma cell lines.

Purpose of the Study:

  • To investigate the expression pattern of UCP2 in liver and hepatoma cells.
  • To explore the regulatory mechanisms of UCP2 expression in hepatocarcinoma.

Main Methods:

  • Differential display screening to identify UCP2 cDNA.
  • Northern blot analysis to assess UCP2 mRNA expression.
  • Cell culture experiments to study UCP2 regulation by serum and phorbol esters.
  • DNA methylation analysis.

Related Experiment Videos

Main Results:

  • UCP2 cDNA was isolated from H4IIE hepatoma cells.
  • UCP2 mRNA was detected in some hepatocarcinoma cell lines, but not in adult hepatocytes.
  • UCP2 expression in H4IIE cells decreased with low serum conditions and was restored by high serum or phorbol esters.
  • Transformed UCP2-expressing cells showed hypomethylation of UCP2.

Conclusions:

  • UCP2 is expressed in certain hepatocarcinoma cell lines, suggesting a role in liver cancer.
  • Serum components and epigenetic modifications (hypomethylation) appear to regulate UCP2 expression in hepatocarcinoma.