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Variability associated with testing shelled corn for fumonisin

T B Whitaker1, M W Trucksess, A S Johansson

  • 1U.S. Department of Agriculture, Agricultural Research Service, North Carolina State University, Raleigh 27695-7625, USA.

Journal of AOAC International
|December 16, 1998
PubMed
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This study quantifies variability in fumonisin testing for corn. Higher fumonisin levels increase test variability, impacting accuracy for mycotoxin analysis.

Area of Science:

  • Agricultural Science
  • Food Safety
  • Analytical Chemistry

Background:

  • Fumonisins are toxic mycotoxins commonly found in corn.
  • Accurate fumonisin measurement is crucial for food safety and agricultural trade.
  • Variability in testing procedures can affect risk assessment and regulatory compliance.

Purpose of the Study:

  • To estimate the variance components associated with sampling, sample preparation, and analysis in fumonisin testing of shelled corn.
  • To determine the relationship between fumonisin concentration and the variance at each step of the test procedure.
  • To compare the overall variability of the fumonisin test procedure with that of aflatoxin testing.

Main Methods:

  • Estimation of variance components for each step of the fumonisin test procedure.

Related Experiment Videos

  • Regression analysis to establish functional relationships between variance and fumonisin concentration.
  • Calculation of coefficients of variation for sampling, sample preparation, and analysis at a specific fumonisin concentration (2 ppm).

    • Main Results:

    • Variance in each test step increases with fumonisin concentration.
    • The relationships between variance and concentration were consistent across different fumonisin types (total, B1, B2, B3).
    • At 2 ppm, coefficients of variation were 16.6% (sampling), 9.1% (preparation), and 9.7% (analysis), with a total of 45% for the procedure.

    Conclusions:

    • The study provides a quantitative understanding of variability in fumonisin testing.
    • The findings highlight the importance of considering concentration-dependent variability in test method validation.
    • The overall variability of the fumonisin test is comparable to that of aflatoxin testing in corn, indicating similar challenges in accurate mycotoxin quantification.