Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thin-layer chromatography-mass spectrometry using activated carbon, surface-assisted laser desorption/ionization

Y C Chen1, J Shiea, J Sunner

  • 1Department of Chemistry and Biochemistry, Montana State University, Bozeman 59717, USA.

Journal of Chromatography. A
|December 16, 1998
PubMed
Summary

This study introduces a rapid method for analyzing compounds using thin-layer chromatography (TLC) coupled with surface-assisted laser desorption ionization (SALDI) mass spectrometry. This technique allows direct analysis from TLC plates, offering a robust approach for various organic compounds and peptides.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Space-charge-dominated mass spectrometry ion sources: Modeling and sensitivity.

Journal of the American Society for Mass Spectrometry·2013
Same author

Role of ion-ion recombination for alkali chloride cluster formation in liquid secondary ion mass spectrometry.

Journal of the American Society for Mass Spectrometry·2013
Same author

Ion transport by viscous gas flow through capillaries.

Journal of the American Society for Mass Spectrometry·2013
Same author

Toxic effects of two brominated flame retardants BDE-47 and BDE-183 on the survival and protein expression of the tubificid Monopylephorus limosus.

Ecotoxicology and environmental safety·2012
Same author

Application of direct electrospray probe to analyze biological compounds and to couple to solid-phase microextraction to detect trace surfactants in aqueous solution.

Analytical chemistry·2011
Same author

Gas chromatography connected to multiple channel electrospray ionization mass spectrometry for the detection of volatile organic compounds.

Analytical chemistry·2011

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Chromatography

Background:

  • Thin-layer chromatography (TLC) is a common separation technique.
  • Direct analysis of TLC separated compounds by mass spectrometry is challenging.
  • Existing methods may suffer from matrix interference and complex sample preparation.

Purpose of the Study:

  • To demonstrate the feasibility of analyzing compounds directly from TLC plates using SALDI-MS.
  • To develop a rapid and robust sample preparation method for TLC-SALDI.
  • To assess the performance characteristics, including detection limits and mass resolution.

Main Methods:

  • Compounds were separated using TLC.
  • The TLC plate surface was prepared by coating with activated carbon particles and glycerol.

Related Experiment Videos

  • Analyte desorption and ionization were achieved using a nitrogen laser coupled to a time-of-flight mass spectrometer (SALDI-MS).

    • Main Results:

    • Direct analysis of TLC-separated compounds by SALDI-MS was successfully demonstrated.
    • A wide range of organic compounds, including peptides, were detected.
    • Low matrix interference was observed, with few intense peaks at low mass in background spectra.
    • A detection limit of approximately 25 ng for bradykinin was achieved.
    • Mass resolution varied between 100 and 500 (FWHM).

    Conclusions:

    • TLC-SALDI-MS is a viable technique for rapid analysis of separated compounds directly from TLC plates.
    • The method offers good sensitivity and low matrix interference.
    • Further optimization is suggested to improve mass resolution and sensitivity.