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Updated: Oct 26, 2026

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
Published on: March 19, 2019
Rapid staining of ultrathin sections with the use of a microwave oven
I Cavusoglu1, Z Kahveci, S A Sirmali
1Uludag University Faculty of Medicine, Department of Histology & Embryology, Bursa, Turkey. Ilkin@uu20.bim.uludag.edu.tr
Abstract:
The microwave oven has many potential applications, ranging from tissue fixation to staining for light and electron microscopy. This study was planned to speed up the staining of ultrathin sections. The first set of grids was stained conventionally with uranyl acetate and Reynold's lead citrate solutions. The other grids were stained with the same solutions by microwave irradiation. The electron micrographs of grids stained using the microwave technique were as satisfactory as the grids stained conventionally. Microwave-treated grids demonstrated more uniform staining and less precipitate. The use of a microwave oven shortened staining time by approximately 38 min.
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