Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antigen retrieval immunohistochemistry used for routinely processed celloidin-embedded human temporal bone sections:

S R Shi1, R J Cote, C R Taylor

  • 1Department of Pathology, University of Southern California School of Medicine, Los Angeles, 90033, USA.

Auris, Nasus, Larynx
|December 16, 1998
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Magnetomicrometry.

Science robotics·2021
Same author

On prosthetic control: A regenerative agonist-antagonist myoneural interface.

Science robotics·2020
Same author

Tonic-clonic seizure after unrecognized unintentional dural puncture.

International journal of obstetric anesthesia·2020
Same author

Issues in using whole slide imaging for diagnostic pathology: "routine" stains, immunohistochemistry and predictive markers.

Biotechnic & histochemistry : official publication of the Biological Stain Commission·2013
Same author

Energy benchmarks for water clusters and ice structures from an embedded many-body expansion.

The Journal of chemical physics·2013
Same author

Clinical pharmacology quality assurance for HIV and related infectious diseases research.

Clinical pharmacology and therapeutics·2013

Antigen retrieval (AR) techniques enhance immunohistochemistry (IHC) for studying the human inner ear. This review addresses technical challenges and suggests improvements for AR-IHC in celloidin-embedded tissues.

Area of Science:

  • Otorhinolaryngology
  • Molecular Biology
  • Histopathology

Background:

  • Immunohistochemistry (IHC) is crucial for understanding inner ear molecular mechanisms.
  • Celloidin embedding is a common method for processing human temporal bone sections.
  • Antigen retrieval (AR) techniques, developed in the early 1990s, have significantly advanced IHC.

Purpose of the Study:

  • To review critical technical issues of the AR technique for celloidin-embedded human temporal bone sections.
  • To provide recommendations for the standardization and further development of AR-IHC.
  • To highlight the utility of AR-IHC in inner ear research using novel data.

Main Methods:

  • Review of existing literature on AR techniques for celloidin-embedded tissues.

Related Experiment Videos

  • Analysis of practical experiences and unpublished data from AR-IHC studies on human temporal bones.
  • Discussion of technical challenges and potential solutions for AR optimization.

    • Main Results:

    • Identification of key technical challenges in applying AR to celloidin-embedded human temporal bone sections.
    • Demonstration of the utility of AR-IHC through examples and unpublished data.
    • Proposal of specific suggestions for improving AR techniques and standardization.

    Conclusions:

    • AR-IHC is a valuable method for molecular-level studies of the human inner ear.
    • Standardization and further development of AR techniques are essential for reliable results.
    • The review provides a foundation for optimizing AR-IHC in celloidin-embedded tissues.