Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple testicular sampling in non-obstructive azoospermia--is it necessary?

R Hauser1, A Botchan, A Amit

  • 1The Institute for the Study of Fertility, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Israel.

Human Reproduction (Oxford, England)
|December 16, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Timing matters: Surgery-to-chemotherapy interval and outcomes in ovarian cancer.

European journal of obstetrics, gynecology, and reproductive biology·2025
Same author

Nutritional status and interventions in hospice: physician assessment of cancer patients.

Journal of human nutrition and dietetics : the official journal of the British Dietetic Association·2018
Same author

Vaccine potential of HLA-A2 epitopes from Leishmania Cysteine Protease Type III (CPC).

Parasite immunology·2017
Same author

Diagnostic utility of brain activity flow patterns analysis in attention deficit hyperactivity disorder.

Psychological medicine·2017
Same author

Human embryonic stem cells carrying an unbalanced translocation demonstrate impaired differentiation into trophoblasts: an in vitro model of human implantation failure.

Molecular human reproduction·2014
Same author

Muscle activity of the erector spinae during Pilates isometric exercises on and off Swiss Ball.

The Journal of sports medicine and physical fitness·2014

Multiple testicular sperm extraction in non-obstructive azoospermia cases increases the chances of retrieving sufficient sperm for fertilization and cryopreservation. Sperm retrieval success was not influenced by biopsy location within the testis.

Area of Science:

  • Reproductive Medicine
  • Urology
  • Andrology

Background:

  • Spermatogenesis can be focal in cases of non-obstructive azoospermia.
  • Identifying viable sperm is crucial for fertility treatments in affected individuals.

Purpose of the Study:

  • To evaluate if multiple testicular biopsies improve sperm retrieval for fertilization and cryopreservation in non-obstructive azoospermia.
  • To determine the significance of biopsy location for sperm retrieval in these patients.

Main Methods:

  • Collected three biopsies from identical locations (rete testis, midline, proximal) in 55 testes from 29 men with non-obstructive azoospermia.
  • Spermatozoa were used for intracytoplasmic sperm injection (ICSI) and cryopreserved.
  • Analyzed sperm presence and quantity in relation to biopsy location.

Related Experiment Videos

Main Results:

  • Spermatozoa were found in 50.9% of testes (62.1% of men).
  • Sperm presence was not significantly influenced by the specific location of the testicular biopsy.
  • When found, sperm were retrieved from one, two, or three locations in 28.6%, 17.9%, and 53.6% of cases, respectively.

Conclusions:

  • Multiple testicular sperm extraction is recommended for non-obstructive azoospermia to enhance diagnostic accuracy and increase sperm yield.
  • This approach improves the likelihood of obtaining sufficient sperm for ICSI and cryopreservation.