Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Olfactory bulb development is altered in small-eye (Sey) mice

T L Dellovade1, D W Pfaff, M Schwanzel-Fukuda

  • 1Laboratory of Neurobiology and Behavior, Rockefeller University, New York, New York 10021, USA. tdellovade@shriver.org

The Journal of Comparative Neurology
|December 16, 1998
PubMed
Summary

The Small-eye mutation in the Pax-6 gene impairs olfactory bulb development in mice. This Pax-6 mutation affects specific neuronal populations and neurotransmitter systems crucial for olfaction.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activating an anterior nucleus gigantocellularis subpopulation triggers emergence from pharmacologically-induced coma in rodents.

Nature communications·2019
Same author

Impacts of stress on reproductive and social behaviors.

Frontiers in neuroendocrinology·2018
Same author

Molecular endocrinology of female reproductive behavior.

Molecular and cellular endocrinology·2017
Same author

Role of Oestrogen α Receptors in Sociosexual Behaviour in Female Rats Housed in a Seminatural Environment.

Journal of neuroendocrinology·2015
Same author

Early histone modifications in the ventromedial hypothalamus and preoptic area following oestradiol administration.

Journal of neuroendocrinology·2013
Same author

CB1 receptor signaling regulates social anxiety and memory.

Genes, brain, and behavior·2013

Area of Science:

  • Neuroscience
  • Genetics
  • Developmental Biology

Background:

  • The Small-eye (Sey) mutation is a semidominant murine mutation caused by a point mutation in the Pax-6 gene.
  • Homozygous Sey mutants exhibit severe defects in eye and olfactory system development, leading to neonatal death.
  • Heterozygous Sey/+ mice display varying degrees of eye abnormalities, including reduced lens size and cataracts.

Purpose of the Study:

  • To investigate the impact of a single mutated Pax-6 allele on olfactory system development in Sey/+ mice.
  • To determine the specific regions within the olfactory system affected by the Sey mutation.
  • To elucidate the neurochemical consequences of the Pax-6 mutation on olfactory bulb interneurons.

Main Methods:

  • Comparative analysis of olfactory bulb volume in Sey/+ mice and wild-type littermates at different ages (42 and 70 days).

Related Experiment Videos

  • Histological examination of olfactory epithelial, vomeronasal organ, and olfactory bulb structures.
  • Immunohistochemical staining for Pax-6, tyrosine hydroxylase (TH), gamma-aminobutyric acid (GABA), calretinin, and calbindin.
  • Dual-labeling immunohistochemistry to identify co-expression of Pax-6 with specific neuronal markers.

    • Main Results:

    • Sey/+ mice showed significantly decreased main olfactory bulb volume, particularly in the glomerular and granule cell layers.
    • Accessory olfactory bulb, olfactory epithelium, and vomeronasal organ development were unaffected.
    • A marked reduction in TH- and GABA-immunoreactive profiles was observed in Sey/+ mice.
    • Pax-6 expression was found in TH-immunoreactive cells and a subpopulation of GABA-immunoreactive cells within the main olfactory bulb.

    Conclusions:

    • Two functional Pax-6 alleles are essential for normal olfactory bulb development.
    • The Pax-6 gene plays a critical role in the development of specific interneuron populations within the main olfactory bulb.
    • The Sey mutation affects olfactory bulb development, potentially through its influence on TH and GABA neurotransmitter systems.