Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Regional differences in protein carboxymethylation in post-mortem human brain

M Goggins1, J M Scott, D G Weir

  • 1Department of Clinical Medicine, University of Dublin, Trinity College, Trinity Centre for Health Sciences, St. James' Hospital, Dublin, Ireland.

Clinical Science (London, England : 1979)
|December 17, 1998
PubMed
Summary

Protein carboxymethylation patterns in the human brain were analyzed using post-mortem tissue. Cortical white matter showed the highest activity, suggesting its vulnerability to hypomethylation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of sulfasalazine and olsalamine on colonic epithelial cell folate content in patients with ulcerative colitis.

Inflammatory bowel diseases·2013
Same author

Homocysteine as a risk factor for cardiovascular and related disease: nutritional implications.

Nutrition research reviews·2008
Same author

Detection of replicative integrity in small colonic biopsies using the BrdUrd comet assay.

British journal of cancer·2003
Same author

The methylenetetrahydrofolate reductase (MTHFR) gene in colorectal cancer: role in tumor development and significance of allelic loss in tumor progression.

International journal of gastrointestinal cancer·2003
Same author

Endocinch therapy for gastro-oesophageal reflux disease: a one year prospective follow up.

Gut·2002
Same author

A prospective study of circulating mutant KRAS2 in the serum of patients with colorectal neoplasia: strong prognostic indicator in postoperative follow up.

Gut·2002

Area of Science:

  • Neuroscience
  • Biochemistry
  • Molecular Biology

Background:

  • Protein carboxymethylation is a crucial post-translational modification.
  • Understanding its distribution in the human brain is vital for neurological research.
  • Post-mortem brain tissue offers a valuable resource for studying methylation patterns.

Purpose of the Study:

  • To investigate the regional distribution of protein carboxymethyltransferase activity in the human brain.
  • To assess the stability of protein carboxymethylation in post-mortem brain tissue.
  • To correlate methylation patterns with potential neurological conditions like vitamin B12 deficiency.

Main Methods:

  • Utilized methyl-3H incorporation to measure protein methylation sites in post-mortem human and pig brain tissue.

Related Experiment Videos

  • Assessed the stability of protein carboxymethylation by sampling tissue at various intervals post-mortem.
  • Analyzed protein carboxymethyltransferase activity across nine distinct regions from 16 human subjects.

    • Main Results:

    • Protein carboxymethylation levels remained stable in post-mortem brain tissue for up to 24 hours.
    • Protein carboxymethyltransferase activity was significantly lower in the cerebellum and highest in cortical white matter.
    • No significant differences were observed in other cortical or subcortical regions.

    Conclusions:

    • Post-mortem human brain tissue is a reliable model for studying protein methylation.
    • Cortical white matter exhibits a higher capacity for protein carboxymethylation compared to other brain regions.
    • The heightened methylation potential in white matter may explain its susceptibility to hypomethylation-related disorders.