Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Poly(ethylene glycol), surface potential and cell fusion

B Maggio, Q F Ahkong, J A Lucy

    The Biochemical Journal
    |September 15, 1976
    PubMed
    Summary

    Poly(ethylene glycol), glycerol, and dimethyl sulfoxide reduce surface potentials in lipid monolayers. This is relevant to understanding how poly(ethylene glycol) causes hen red blood cell fusion.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hexagonal phase with ordered acyl chains formed by a short chain asymmetric ceramide.

    Colloids and surfaces. B, Biointerfaces·2016
    Same author

    Surface interactions, thermodynamics and topography of binary monolayers of Insulin with dipalmitoylphosphatidylcholine and 1-palmitoyl-2-oleoylphosphatidylcholine at the air/water interface.

    Journal of colloid and interface science·2015
    Same author

    Rheological properties of regular insulin and aspart insulin Langmuir monolayers at the air/water interface: condensing effect of Zn2+ in the subphase.

    Colloids and surfaces. B, Biointerfaces·2013
    Same author

    Controlled lateral packing of insulin monolayers influences neuron polarization in solid-supported cultures.

    Colloids and surfaces. B, Biointerfaces·2013
    Same author

    A 3-day randomised clinical study investigating the efficacy of two toothpastes, designed to occlude dentine tubules, for the treatment of dentine hypersensitivity.

    Journal of dentistry·2012
    Same author

    A preliminary investigation into the use of denture adhesives combined with dietary advice to improve diets in complete denture wearers.

    Journal of dentistry·2012

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Surface Chemistry

    Background:

    • Phospholipids like phosphatidylcholine and phosphatidylethanolamine are key components of cell membranes.
    • Surface potential plays a critical role in cell membrane interactions and fusion processes.
    • Polyols and polymers are known to influence membrane properties.

    Purpose of the Study:

    • To investigate the effect of poly(ethylene glycol) (PEG), glycerol, and dimethyl sulfoxide (DMSO) on the surface potentials of lipid monolayers.
    • To explore the relationship between these surface potential changes and the phenomenon of hen erythrocyte fusion induced by PEG.

    Main Methods:

    • Formation of lipid monolayers using phosphatidylcholine and phosphatidylethanolamine.
    • Measurement of surface potentials of these monolayers in the presence of different solutes.
    • Observation and analysis of hen erythrocyte fusion induced by poly(ethylene glycol).

    Main Results:

    • Poly(ethylene glycol), glycerol, and dimethyl sulfoxide significantly decreased the surface potentials of both phosphatidylcholine and phosphatidylethanolamine monolayers.
    • The observed decrease in surface potential is correlated with the fusogenic effects of poly(ethylene glycol) on hen erythrocytes.

    Conclusions:

    • The reduction of surface potential by PEG, glycerol, and DMSO is a key factor influencing membrane fusion.
    • Understanding these surface potential alterations provides insights into the mechanism of PEG-induced cell fusion.

    Related Experiment Videos