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Influenza virus hemagglutinin stimulates the protein kinase C activity of human polymorphonuclear leucocytes

D J Arora1, N Gasse

  • 1Virology Research Center, Institut Armand-Frappier, Laval, Québec, Canada.

Archives of Virology
|December 18, 1998
PubMed

Insights

Influenza virus and its hemagglutinin activate protein kinase C (PKC) in human polymorphonuclear leukocytes (PMNL). This enzyme activation involves translocation and suggests the involvement of specific PKC isoforms.

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Human polymorphonuclear leukocytes (PMNL) play a crucial role in the innate immune response.
  • Influenza virus infections can modulate immune cell function.

Purpose of the Study:

  • To investigate the effect of influenza virus on protein kinase C (PKC) activity in human PMNL.
  • To determine the cellular localization and potential isoforms of PKC involved.

Main Methods:

  • Incubation of human PMNL with influenza virus (A/USSR/90/77 H1N1) or its hemagglutinin.
  • Measurement of PKC activity and assessment of enzyme translocation.
  • Use of specific inhibitors to probe PKC isoform involvement.

Main Results:

  • Influenza virus and hemagglutinin significantly increased PKC activity in PMNL.
  • Activated PKC translocated from the cytosol to the cellular membrane.
  • Calcium-dependent activity and inhibition by a specific inhibitor indicated involvement of alpha and/or beta PKC isoforms.

Conclusions:

  • Influenza virus modulates human PMNL function through PKC activation.
  • PKC activation is associated with kinase translocation and calcium-dependent signaling.
  • Alpha and/or beta PKC isoforms are likely involved in the cellular response to influenza virus.

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