Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chemoattractant-induced lamellipod extension

M Bailly1, J S Condeelis, J E Segall

  • 1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

Microscopy Research and Technique
|December 19, 1998
PubMed
Summary

Epidermal growth factor (EGF) stimulates breast cancer cell movement by promoting actin polymerization and lamellipod extension. This process is crucial for cell invasion and metastasis, highlighting EGF

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whole-exome sequencing in patients with maturation arrest: a potential additional diagnostic tool for prevention of recurrent negative testicular sperm extraction outcomes.

Human reproduction (Oxford, England)·2022
Same author

[Comparative analysis of the financing of follow-up and rehabilitation care institutions and public mental health institutions: Application for innovative and expensive drugs].

Annales pharmaceutiques francaises·2021
Same author

Direct measurement of vertical forces shows correlation between mechanical activity and proteolytic ability of invadopodia.

Science advances·2020
Same author

Clinical evaluation of General Electric new Swiftscan solution in bone scintigraphy on NaI-camera: A head to head comparison with Siemens Symbia.

PloS one·2019
Same author

Disparities in reproductive outcomes according to the endometrial preparation protocol in frozen embryo transfer : The risk of early pregnancy loss in frozen embryo transfer cycles.

Journal of assisted reproduction and genetics·2017
Same author

[Male age in assisted reproductive technologies: Is there a limit?]

Gynecologie, obstetrique & fertilite·2016

Area of Science:

  • Cell Biology
  • Cancer Research
  • Biochemistry

Background:

  • Mammary adenocarcinoma cells (MTLn3) exhibit chemotaxis towards epidermal growth factor (EGF).
  • EGF-induced cell motility is implicated in breast cancer invasion and metastasis.
  • EGF stimulation triggers the extension of actin-rich lamellipodia in MTLn3 cells.

Purpose of the Study:

  • To analyze chemoattractant-stimulated protrusion in EGF-stimulated MTLn3 cells.
  • To investigate the role of actin polymerization and cell-substratum interactions in lamellipod extension.
  • To understand the coordination of focal contact dynamics with lamellipod extension for polarized motility.

Main Methods:

  • Stimulation of MTLn3 cells with epidermal growth factor (EGF).
  • Microscopic analysis of lamellipod structure and actin polymerization.

Related Experiment Videos

  • Assessment of cell-substratum adhesion and focal contact turnover.

    • Main Results:

    • EGF stimulation induces lamellipod extension, dependent on actin polymerization at the cell border.
    • Lamellipod extension is independent of substratum interaction, but stabilization requires adhesion.
    • Reduced tyrosine phosphorylation in existing focal contacts and increased new contacts correlate with protrusion.

    Conclusions:

    • EGF-driven lamellipod extension is a key mechanism for directed cell movement in response to chemoattractants.
    • The interplay between actin dynamics, adhesion, and focal contact remodeling facilitates polarized cell motility.
    • Understanding these mechanisms is vital for targeting breast cancer invasion and metastasis.