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Related Experiment Videos

Serotyping scheme for Staphylococcus aureus isolated from cows with mastitis

A Guidry1, A Fattom, A Patel

  • 1Immunology and Disease Resistance Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, USA.

American Journal of Veterinary Research
|December 19, 1998
PubMed
Summary

A new Staphylococcus aureus serotyping antiserum (336) identified previously untypable strains from bovine mastitis milk samples. This advancement significantly improves Staphylococcus aureus typing accuracy in both US and European dairy herds.

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Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis
  • Dairy Science

Background:

  • Bovine mastitis is a significant economic challenge in the dairy industry.
  • Staphylococcus aureus is a primary causative agent of bovine mastitis.
  • Current capsular serotyping methods for Staphylococcus aureus (types 5 and 8) are insufficient for comprehensive strain identification.

Purpose of the Study:

  • To identify Staphylococcus aureus capsular serotypes not currently typable by serotypes 5 and 8.
  • To evaluate the efficacy of a newly developed serotyping antiserum (336) in identifying Staphylococcus aureus strains from bovine mastitis cases.

Main Methods:

  • Collected milk samples from dairy herds in the US and Europe experiencing mastitis.
  • Performed capsular serotyping using direct cell agglutination and immunoprecipitation.

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  • Utilized antisera specific for capsular types 5 and 8, alongside a new antiserum (336).

    • Main Results:

    • In the US, Staphylococcus aureus types 5 and 8 represented 18% and 23% of isolates, respectively, with type 336 accounting for 59%.
    • In Europe, type 5 was 34%, type 8 was 34%, type 336 was 30%, and only 2% were nontypable.
    • The addition of antiserum 336 enabled typing of 100% of US isolates and 98% of European isolates.

    Conclusions:

    • Serotypes 5 and 8 alone are inadequate for typing Staphylococcus aureus from bovine mastitis milk.
    • The inclusion of serotyping antiserum 336 significantly enhances the ability to type Staphylococcus aureus isolates.
    • This improved typing scheme is crucial for developing a more effective Staphylococcus aureus vaccine for dairy cattle.