Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Overlapping and non-overlapping Ptch2 expression with Shh during mouse embryogenesis

J Motoyama1, H Heng, M A Crackower

  • 1Program in Developmental Biology, Research Institute, The Hospital for Sick Children, 555 University Avenue, Toronto, Ont. M5G 1X8, Canada. motoyama@sickkids.on.ca

Mechanisms of Development
|December 22, 1998
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

2023 Undetectable = Untransmittable global policy roundtable report: a multistakeholder perspective on Undetectable = Untransmittable research and policy priorities.

Sexual health·2024
Same author

Global evidence, impact and implementation of U=U.

Sexual health·2023
Same author

Extracellular matrix degradation pathways and fatty acid metabolism regulate distinct pulmonary vascular cell types in pulmonary arterial hypertension.

Pulmonary circulation·2021
Same author

The implementation of rifapentine and isoniazid (3HP) in two remote Arctic communities with a predominantly Inuit population, the Taima TB 3HP study.

International journal of circumpolar health·2020
Same author

STATEMENT ON PEDIATRIC TRAVELLERS: Committee to Advise on Tropical Medicine and Travel.

Canada communicable disease report = Releve des maladies transmissibles au Canada·2019
Same author

Statement on International Adoption: Committee to Advise on Tropical Medicine and Travel.

Canada communicable disease report = Releve des maladies transmissibles au Canada·2019

Mouse patched homologue 2 (Ptch2) functions in Sonic hedgehog (Shh) signaling. Ptch2 is expressed in various developing tissues, often near Shh-producing cells, suggesting a role in Shh pathway regulation.

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Patched (Ptch) proteins are negative regulators of Hedgehog signaling.
  • Vertebrate patched homologues, including Ptch and Ptch2, are implicated as Sonic hedgehog (Shh) receptors.
  • Ptch and Ptch2 may have distinct roles in Shh signaling pathways.

Purpose of the Study:

  • To determine the chromosomal localization of the mouse Ptch2 gene.
  • To analyze the expression patterns of Ptch2 during mouse development.
  • To investigate the relationship between Ptch2 and Shh expression in developing tissues.

Main Methods:

  • Chromosomal localization analysis.
  • Quantitative expression analysis (comparing Ptch and Ptch2 levels).

Related Experiment Videos

  • In situ expression analysis in developing mouse embryos and specific tissues (CNS, tooth, hair, whisker, nasal gland, eyelids).

    • Main Results:

    • Ptch2 expression levels are significantly lower than Ptch levels throughout mouse development.
    • Ptch2 is co-expressed with Shh in the developing central nervous system (CNS), nasal gland, and eyelids.
    • Ptch2 expression is observed in both Shh-producing and Shh-nonproducing cells during development.

    Conclusions:

    • Ptch2 exhibits a distinct expression pattern compared to Ptch during mouse development.
    • The co-expression of Ptch2 with Shh in various tissues suggests its involvement in Shh signal reception or modulation.
    • Ptch2's expression in both Shh-producing and non-producing cells indicates a complex regulatory role in the Hedgehog pathway.