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Limited proteolysis of a trypanosomal hypoxanthine phosphoribosyltransferase yields crystals that diffract X-rays to
R Nieves-Alicea1, P J Focia, S P Craig
1Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, San Juan, PR 00936-5067, USA.
Biochimica Et Biophysica Acta
|December 22, 1998
Abstract:
Two crystal forms of the hypoxanthine phosphoribosyltransferase from Trypanosoma cruzi were grown and characterized. Proteolytic modification at the C-terminus of the recombinant enzyme yielded monoclinic crystals that diffract X-rays to higher resolution than the original, trigonal crystal form. Data from the monoclinic crystal form enabled determination of the crystal structure for the trypanosomal HPRT to 1.4 A resolution.