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Updated: Aug 14, 2026

2D and 3D Chromosome Painting in Malaria Mosquitoes
Published on: January 7, 2014
Sperm segregation analysis of a complex chromosome rearrangement, 2;22;11, by whole chromosome painting
P Cifuentes1, J Navarro, L Míguez
1Unitat de Biologia, Facultat de Medicina, Departament de Biologia Cel.lular i Fisiologia, Universitat Autònoma de Barcelona, (Spain). biolmed@cc.uab.es
Abstract:
Using the human sperm-hamster oocyte fusion technique and whole chromosome painting, we studied sperm chromosome segregation in a male heterozygous for a complex chromosome rearrangement, 46,XY,-2, +der(2)t(2;11)(q13; q23),-11,+der(11)t(11;22)(q23;q11.2),-22, +der(22)t(2;22)(q13; q11.2). A total of 208 sperm complements were analyzed. The frequency of sperm carrying a normal or a balanced complement was 13.5% (9.62% and 3.85%, respectively). The frequency of unbalanced sperm was 86.5% (64.9% from 3:3 segregation, including 30 different types; 20.7% from 4:2 segregation, including 21 different types; and 0.96% from 5:1 segregation, including 2 different types). The sex ratio, determined in 134 sperm complements, did not differ from the expected 1:1 ratio. The results obtained in this study are compatible with the formation, during the synaptic process, of a complex hexavalent figure involving chromosomes 2, 11, and 22. The behavior and segregation of this complex figure would explain the high frequency (86.5%) of unbalanced complements observed in this carrier.
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