Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Time-resolved crystallography

K Moffat1

  • 1Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637, USA. moffat@cars.uchicago.edu

Acta Crystallographica. Section A, Foundations of Crystallography
|December 22, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scintillation light detection in the 6-m drift-length ProtoDUNE Dual Phase liquid argon TPC.

The European physical journal. C, Particles and fields·2022
Same author

Prospects for beyond the Standard Model physics searches at the Deep Underground Neutrino Experiment: DUNE Collaboration.

The European physical journal. C, Particles and fields·2021
Same author

McMaster RARE-Bestpractices clinical practice guideline on diagnosis and management of the catastrophic antiphospholipid syndrome.

Journal of thrombosis and haemostasis : JTH·2018
Same author

Cooperative elastic switching vs. laser heating in [Fe(phen)2(NCS)2] spin-crossover crystals excited by a laser pulse.

CrystEngComm·2017
Same author

Hemodialysis for the treatment of dabigatran-associated bleeding: a case report and systematic review.

Journal of thrombosis and haemostasis : JTH·2015
Same author

Out-of-equilibrium dynamics of photoexcited spin-state concentration waves.

Faraday discussions·2015
Same journal

Report of the Executive Committee for 2006.

Acta crystallographica. Section A, Foundations of crystallography·2020
Same journal

Spin line groups.

Acta crystallographica. Section A, Foundations of crystallography·2013
Same journal

Distribution rules of systematic absences on the Conway topograph and their application to powder auto-indexing.

Acta crystallographica. Section A, Foundations of crystallography·2013
Same journal

Platonic solids generate their four-dimensional analogues.

Acta crystallographica. Section A, Foundations of crystallography·2013
Same journal

C70, C80, C90 and carbon nanotubes by breaking of the icosahedral symmetry of C60.

Acta crystallographica. Section A, Foundations of crystallography·2013
Same journal

Comparative study of X-ray charge-density data on CoSb3.

Acta crystallographica. Section A, Foundations of crystallography·2013
See all related articles

Time-resolved crystallography now captures molecular dynamics across various timescales, from seconds to picoseconds. This technique reveals time-dependent structural changes in macromolecular systems, advancing crystallography beyond static snapshots.

Area of Science:

  • Biophysics
  • Structural Biology
  • Crystallography

Background:

  • Time-resolved crystallography (TRX) has advanced significantly, enabling studies from seconds to picoseconds.
  • Recent progress has focused on macromolecular systems, with improved experimental strategies to minimize artifacts.

Purpose of the Study:

  • To review the application and advancements of time-resolved crystallography.
  • To highlight strategies for successful TRX experiments and identify remaining challenges.

Main Methods:

  • Application of time-resolved crystallography across diverse timescales (seconds, milliseconds, nanoseconds, picoseconds).
  • Development and implementation of experimental strategies to mitigate artifacts in crystallographic studies.
  • Analysis of time-dependent X-ray diffraction data.

Related Experiment Videos

Main Results:

  • Successful application of TRX on various macromolecular systems.
  • Identification of strategies for artifact reduction in TRX experiments.
  • Demonstration that time can be considered a fourth dimension in crystallography.

Conclusions:

  • Time-resolved crystallography is a powerful technique for studying dynamic molecular processes.
  • Crystallography is evolving from a static to a dynamic structural biology method.
  • Computational methods are crucial for extracting time-independent structures from TRX data.