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[A comparative analysis of energy process inhibitors on the efficiency of phage infection in staphylococci]
1Dniepropetrovsk State University.
Abstract:
The study of the effect of KCN, DCCD and CCCP as inhibitors of the energy yielding processes showed that the efficacy of phage infection depended on respiration, proton ATPase, and proton electrochemical potential of hydrogen ions. There was a 49.5-68.0% decrease of the efficacy of phage infection after addition of the above mentioned inhibitors at the period of the contact of cells with bacteriophages at the stage of the phage nucleic acid transfer. The Embden-Meyerhof-Parnas route inhibitors NaF and CH2ICOOH less affected the efficacy of phage infection. The same effect was observed during addition of Na3AsO4 as the ATP synthesis inhibitor. This efficacy decrease was probably due the inhibition of the processes of the substrate level phosphorylation and the deplete of the intracellular ATP content.
Insights
Phage infection efficacy relies on cellular respiration and proton gradients. Inhibiting these energy processes significantly reduced infection success, highlighting their crucial role.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- Bacteriophage infection is a complex process influenced by host cell physiology.
- Energy metabolism plays a critical role in various stages of viral replication.
Purpose of the Study:
- To investigate the impact of energy metabolism inhibitors on bacteriophage infection efficacy.
- To determine the specific energy-dependent processes essential for phage infection.
Main Methods:
- Utilizing inhibitors of respiration (KCN), proton ATPase (DCCD), and proton electrochemical potential (CCCP).
- Assessing phage infection efficacy following treatment with Embden-Meyerhof-Parnas pathway inhibitors (NaF, CH2ICOOH) and an ATP synthesis inhibitor (Na3AsO4).
- Quantifying the reduction in phage infection rates under different inhibitory conditions.
Main Results:
- Inhibitors of respiration, proton ATPase, and proton electrochemical potential decreased phage infection efficacy by 49.5-68.0%.
- Embden-Meyerhof-Parnas pathway inhibitors and Na3AsO4 had a lesser effect on infection efficacy.
- The observed decrease in efficacy correlated with the inhibition of substrate-level phosphorylation and ATP depletion.
Conclusions:
- Cellular respiration, proton ATPase activity, and proton electrochemical potential are vital for efficient phage infection.
- Energy-dependent processes, particularly those linked to oxidative phosphorylation, are critical during the phage nucleic acid transfer stage.
- Disruption of cellular energy production significantly impairs bacteriophage infectivity.