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Globin chain synthesis analysis by high performance liquid chromatography in the screening of thalassemia syndromes
R Galanello1, S Satta, M G Pirroni
1Istituto di Clinica e Biologia dell'Età Evolutiva, Università degli Studi di Cagliari Ospedale Regionale Microcitemie, Italia.
Hemoglobin
|December 22, 1998
Abstract:
We applied reversed phase high performance liquid chromatography for globin chain synthesis analysis in screening for beta-thalassemia. The alpha/non-alpha-globin chain synthesis ratios have been determined in alpha-, beta-, and deltabeta-thalassemia carriers using the classical carboxymethyl cellulose chromatography as the reference method. Reversed phase high performance liquid chromatography is fast, accurate, and reproducible, and may be a suitable alternative for the traditional carboxymethyl cellulose chromatography.