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Related Experiment Videos

Zebrafish vimentin: molecular characterization, assembly properties and developmental expression

J Cerdà1, M Conrad, J Markl

  • 1Division of Cell Biology, German Cancer Research Center, Heidelberg.

European Journal of Cell Biology
|December 22, 1998
PubMed
Summary
This summary is machine-generated.

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Zebrafish vimentin (intermediate filament protein) assembly is temperature-dependent, with optimal function around 28°C. This discovery enables new research into vimentin

Area of Science:

  • Cell Biology
  • Biochemistry
  • Developmental Biology

Background:

  • Intermediate filaments (IFs) are crucial cytoskeletal components.
  • Vimentin is a key IF protein involved in tissue structure and integrity.
  • Zebrafish (Danio rerio) offer a powerful vertebrate model for genetic and cell biological studies.

Purpose of the Study:

  • To isolate and characterize zebrafish vimentin for experimental use.
  • To investigate the assembly properties of zebrafish vimentin.
  • To explore the potential of zebrafish vimentin in rescue experiments and its role in tissue development.

Main Methods:

  • cDNA cloning and sequencing of zebrafish vimentin.
  • Recombinant protein expression and in vitro assembly studies.

Related Experiment Videos

  • Viscometry to determine optimal assembly temperature.
  • Mixing experiments with trout vimentin to assess functional rescue.

    • Main Results:

    • Zebrafish vimentin cDNA shows high sequence identity to human vimentin.
    • Optimal assembly of zebrafish vimentin occurs between 21-34°C, with a peak around 28°C.
    • Abnormal filament formation and aggregation observed at temperatures below 21°C and above 37°C.
    • Zebrafish vimentin partially rescued temperature-dependent assembly defects in trout vimentin.
    • Vimentin is abundant in the zebrafish lens, retina, and nervous system during development.

    Conclusions:

    • Zebrafish vimentin exhibits unique temperature-dependent assembly characteristics.
    • Its ability to rescue other vimentins suggests conserved functional mechanisms.
    • The findings support the hypothesis that vimentin is vital for functional adult tissue generation.
    • Zebrafish provide a valuable model for studying vimentin's role in eye and brain development, particularly the retina, using both cell biology and genetics.