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Related Experiment Videos

Albumin-binding surfaces: in vitro activity

C D McFarland1, C De Filippis, M Jenkins

  • 1Cooperative Research Centre for Cardiac Technology, CSIRO Molecular Science, Sydney Laboratory, North Ryde, NSW, Australia.

Journal of Biomaterials Science. Polymer Edition
|December 22, 1998
PubMed
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Immobilized monoclonal antibodies (Mabs) can control biomaterial surface interactions. Anti-albumin Mabs significantly reduce platelet and fibrinogen adhesion, demonstrating Mabs

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Immunology

Background:

  • Immobilized monoclonal antibodies (Mabs) are crucial for controlling molecular interactions on solid surfaces in complex biological mixtures.
  • Rational biomaterial design aims to direct biological responses through surface modification.

Purpose of the Study:

  • To investigate the use of immobilized Mabs to modulate protein and cell adhesion on biomaterial surfaces.
  • To determine the effect of specific Mabs, such as anti-albumin, on platelet and fibrinogen adsorption.
  • To explore the creation of patterned surfaces with controlled adhesive and non-adhesive regions.

Main Methods:

  • Immobilization of monoclonal antibodies (Mabs) onto various substrates.
  • Assessment of fibrinogen adsorption from plasma using techniques like the Vroman effect.

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  • Evaluation of platelet adhesion from platelet-rich plasma and whole blood under static and flow conditions.
  • Comparison of Mab-treated surfaces with untreated controls.

    • Main Results:

    • Surface-bound anti-albumin Mabs significantly reduced fibrinogen adsorption by 64% compared to controls.
    • Immobilization of anti-human serum albumin (HSA) Mabs markedly decreased platelet adhesion on fluorinated ethylene propylene (FEP) surfaces (p < 0.0001).
    • Reduced platelet adhesion was also observed in Mab-treated polyurethane tubing under flow conditions (p=0.034).

    Conclusions:

    • Monoclonal antibodies can be strategically immobilized to engineer biomaterial surfaces with tailored adhesive properties.
    • Albumin-mediated effects are significant in controlling protein and platelet interactions.
    • This approach offers a method for creating surfaces that actively manage cellular and protein responses in biological environments.