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HNS, a nuclear-cytoplasmic shuttling sequence in HuR

X C Fan1, J A Steitz

  • 1Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, Boyer Center for Molecular Medicine, New Haven, CT 06536, USA.

Proceedings of the National Academy of Sciences of the United States of America
|December 23, 1998
PubMed
Summary

Researchers discovered a novel shuttling sequence in the RNA-binding protein HuR. This sequence facilitates nuclear export of messenger RNA (mRNA) and may protect it during transport.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Nuclear transport is crucial for protein and RNA localization within cells.
  • Classical nuclear localization signals and nuclear export signals mediate most nuclear transport.
  • Shuttling sequences enable bidirectional transport of RNA-binding proteins, such as HuR, between the nucleus and cytoplasm.

Purpose of the Study:

  • To identify and characterize the shuttling mechanism of the RNA-binding protein HuR.
  • To investigate the role of HuR in mRNA export and stabilization.

Main Methods:

  • Identification of a novel shuttling sequence within HuR.
  • Analysis of transcription-dependent nuclear localization signal activity.
  • Investigation of HuR's role in binding and exporting AU-rich element-containing mRNAs.

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Main Results:

  • A novel shuttling sequence was identified in HuR.
  • This sequence exhibits transcription-dependent nuclear localization signal activity.
  • HuR binds AU-rich element-containing mRNAs in the nucleus and facilitates their export.

Conclusions:

  • HuR possesses a unique shuttling sequence that mediates bidirectional nucleocytoplasmic transport.
  • This sequence contributes to the export of specific mRNAs to the cytoplasm.
  • HuR may protect mRNAs during nuclear export, ensuring their stability.