[Metastasis of sarcoma 45 and 536 following different methods of inoculation]

Intertesticular inoculation to rats of 1 ml of sarcoma suspension was accompanied by a rapid growth of the tumour at the site of inoculation and the spread of the tumour nodes to the omentum majus, porta hepatis, mesentery and the abdominal walls. After intraperitoneal inoculation the spread of the tumour nodes was similar. In these experiments animals died in 13-15 days. One month after the intratesticular inoculation of 0.2 ml of the tumour suspension-lung metastases were revealed in 74% of the animals with sarcoma-45 and in 90% of those with sarcoma-536. It was shown that tumour cells appeared in the blood stream already during the inoculation. However, laying of lung metastases occurred only after the formation of a primary tumour node at the site of transplantation. A conclusion was drawn that this system was a suitable experimental model for the study of the lung metastases development in rats.