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Decrease in Ca-ATPase activity in aged synaptosomal membranes is not associated with changes in fatty acyl chain

Z Qin1, A Zaidi, J Gao

  • 1Department of Biochemistry, Cell and Molecular Biology, University of Kansas 66045-2106, USA.

Mechanisms of Ageing and Development
|December 23, 1998
PubMed
Summary
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Aging reduces synaptic plasma membrane Ca2(+)-ATPase (PMCA) activity in rat brains. Lipid peroxidation and membrane fluidity remain unchanged, suggesting direct protein modification contributes to age-related PMCA decline.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Gerontology

Background:

  • Synaptic plasma membrane Ca2(+)-ATPase (PMCA) activity declines with age.
  • Lipid peroxidation (LPO) and membrane dynamics are potential factors influencing PMCA function.

Purpose of the Study:

  • To investigate the relationship between LPO, synaptosomal membrane dynamics, and age-related PMCA activity decline in rat brains.

Main Methods:

  • Isolated synaptosomal membranes from aged rats.
  • Measured PMCA enzymatic activity.
  • Analyzed LPO breakdown products using HPLC.
  • Assessed membrane fluidity with electron paramagnetic resonance (EPR) spectroscopy.

Main Results:

  • PMCA activity decreased by approximately 35% in aged rats.

Related Experiment Videos

  • No significant age-related increase in LPO breakdown products (aldehydes) was observed.
  • Bilayer fluidity and lipid dynamics (boundary and bulk lipids) remained unaltered with age.

    • Conclusions:

    • Age-related decline in PMCA activity is not explained by changes in LPO or membrane fluidity.
    • Direct modification of the PMCA protein is the likely cause of age-related functional decline.