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Updated: Oct 17, 2026

Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana
Published on: April 19, 2014
Tissue-specific expression of genes encoding isoforms of the mitochondrial ATPase beta subunit in Nicotiana
E Lalanne1, C Mathieu, F Vedel
1Institut de Biotechnologie des Plantes, ERS CNRS 569, Université Paris-Sud, Orsay, France.
Abstract:
We report here that the catalytic beta subunit of the mitochondrial ATPase/ATP synthase is encoded by a small multigenic family in the diploid tobacco Nicotiana sylvestris (nsatp2 genes). cDNAs and genes corresponding to the beta1, beta2 and beta3 (pollen specific) isoforms previously detected by 2D-SDS PAGE were isolated. Nsatp2.1 and nsatp2.2 transcripts were found in all vegetative and reproductive tissues analysed. In contrast, nsatp2.3 transcripts were found exclusively in bicellular pollen. As a whole, steady-state transcript levels of nuclear nsatp2 and mitochondrial atp1 genes were found to be closely correlated.
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