Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A DNA polymerase stop assay for G-quadruplex-interactive compounds

H Han1, L H Hurley, M Salazar

  • 1Program in Molecular Biology, Division of Medicinal Chemistry and Drug Dynamics Institute, College of Pharmacy, The University of Texas at Austin, Austin, TX 78712, USA. m-salazar@mail.utexas.edu

Nucleic Acids Research
|December 24, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Prognostic impact of lymphovascular invasion and efficacy of adjuvant therapy in patients with non-metastatic clear cell renal cell carcinoma: a single-center retrospective cohort study].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2026
Same author

Competitive enzyme-linked immunosorbent assay to quantify hepcidin in horse serum.

Journal of equine veterinary science·2026
Same author

[Prognostic value of lymph node dissection in radical nephrectomy for cN0M0 renal cell carcinoma].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2026
Same author

Early detection of renal cell carcinoma: a novel cell-free DNA fragmentomics-based liquid biopsy assay.

ESMO open·2025
Same author

[Dynamic distribution and clearance of <sup>99m</sup>Tc-DTPA in brain extracellular space].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2025
Same author

[<i>Gandou Bushen</i> Decoction improves spermatogenesis and promotes spermatogenic cell proliferation in Wilson disease TX mice by activating testicular ERK signaling pathway].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2024

We developed a new assay to find G-quadruplex-interactive compounds. This method uses DNA polymerase arrest to detect agents like BSU-1051 that stabilize G-quadruplex DNA, aiding drug discovery.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • G-rich DNA sequences can form G-quadruplex structures.
  • These structures can impede DNA synthesis by DNA polymerases.
  • Identifying compounds that interact with G-quadruplexes is crucial for therapeutic development.

Purpose of the Study:

  • To develop and characterize a novel assay for screening G-quadruplex-interactive compounds.
  • To investigate the effect of the compound BSU-1051 on DNA synthesis in the presence of G-rich templates.
  • To evaluate the utility of a polymerase arrest assay for identifying potential therapeutic agents.

Main Methods:

  • Utilized Taq DNA polymerase for DNA synthesis.
  • Employed G-rich DNA templates (TTGGGG or TTAGGG repeats).

Related Experiment Videos

  • Assessed the impact of the G-quadruplex binding compound BSU-1051 and potassium ions (K+) on DNA synthesis arrest.

    • Main Results:

    • BSU-1051 significantly enhanced the arrest of DNA synthesis on G-rich templates.
    • This enhancement was attributed to the stabilization of intramolecular G-quadruplex structures.
    • The findings support BSU-1051's role in modulating telomerase activity via telomeric G-quadruplex stabilization.

    Conclusions:

    • A polymerase arrest assay is a sensitive method for detecting G-quadruplex-interactive agents.
    • BSU-1051 stabilizes G-quadruplex DNA, impacting DNA synthesis.
    • This assay holds potential for screening compounds with clinical utility in targeting G-quadruplex structures.