Related Experiment Video
Updated: Sep 13, 2026
Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis
Published on: May 26, 2021
[Effects of composite blood-activating decoction on adhesive function of bone marrow stroma cells in radiation
Objective:
To evaluate the effect of commonly used "blood activating and stasis-removing" Chinese herbal drugs on adhesive function of bone marrow stroma cells in acute radiation injured mice.
Methods:
After irradiation by 8 Gy 60Co gamma-ray, each mouse was intraperitoneally injected immediately with 0.2 ml 100% composite blood-activating decoction (CBAD) twice a day for 6 days. On the 7th day, the femura were taken and the bone karyocyte (BMC) suspension was made. According to long term bone marrow culture procedure, on the 12th day, the fibroblastic colony forming units were counted, and on the 28th day, the adhesion of culture stroma cells to normal murine BMC was observed.
Results:
The adhesion of culture stroma cells to normal murine BMC was 67.8 +/- 17.2% in normal group, while in CBAD group, it was 55.8 +/- 10.5%. The difference was insignificant (P > 0.05). In the control group, the value was 47.7 +/- 13.6%, which was significantly lower than that in normal group (P < 0.02).
Conclusion:
The commonly used "blood-activating and stasis-removing" Chinese herbal drugs can enhance the adhesive function of bone marrow stroma cells in acute radiation injured mice.
