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Correlation between expression of mdr-1 gene and oncogenes in human promyelocytic leukemic HL60 cell line and

W D Zhou¹, H Q Zhang, M Fang

¹Department of Biology, Beijing Normal University, China.

Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica

|September 1, 1996

Abstract

Aim:

To study the relationship between expression of oncogenes and multiple drug resistant (MDR) phenotype.

Methods:

The drug resistant level of HL60 cell line and its sublines were determined with flow cytometry. RNA Dot blot hybridization was used to identify the expression of oncogenes and mdr-1 gene.

Results:

The expression of mdr-1 gene was in the opposite relation with c-myc expression, but in the positive relation with c-H-ras gene expression in the multiple drug resistant cell lines. In non-MDR cell line HL60/RA, the expression levels of mdr-1, c-myc, and c-H-ras were the same as HL60 parental cells.

Conclusion:

Multiple drug resistance is related to not only mdr-1 expression, but also some oncogenes expression level.

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