[Studies on selectivity of recognizing factor for rmf promoter]

The truncted DNA template carrying rmf gene promoter was transcripted by E. coli RNA polymorase holoenzyme (E sigma) reconstituted with core enzyme (alpha 2 beta beta') and sigma 70 or sigma 38 in vitro. The initional site of the transcription of rmf was confirmed with different restriction endonuclease. rmf promoter can be recognized by E sigma 70 but not E sigma 38. The suitable temperature for in vitro transcription was 37 degrees C, NaCl concentration was 50 mmol/L.