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[Morphogenesis of AcMNPV nucleocapsid]

J Chen1, J Teng, Z Zhai

  • 1College of Life Science, Peking University, Beijing.

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|April 1, 1997
PubMed
Summary

This study details the AcMNPV nucleocapsid assembly in sf9 cells. Viral DNA and capsid proteins form unique tubular structures, leading to mature, enveloped virions.

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Area of Science:

  • Molecular Virology
  • Cell Biology
  • Insect Pathology

Background:

  • Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a significant insect pathogen.
  • Understanding viral morphogenesis is crucial for baculovirus research and applications.
  • The role of the polyhedrin gene in AcMNPV assembly requires further elucidation.

Purpose of the Study:

  • To investigate the nucleocapsid morphogenetic process of AcMNPV in sf9 cells.
  • To characterize the assembly pathway in the absence of the polyhedrin gene.

Main Methods:

  • Electron microscopy was used to visualize the structural changes during viral assembly.
  • sf9 cells were infected with a polyhedrin gene-deleted AcMNPV mutant.
  • Analysis focused on the sequential formation of viral structures within the cell nucleus.

Main Results:

  • Capsid proteins initially formed hollow, fascicular tubes (34nm diameter) in the nucleus.
  • Viral DNA entered these tubes, creating electron-dense, solid structures.
  • These structures segmented into bacilliform nucleocapsids (34 x 26 nm), which were subsequently enveloped.

Conclusions:

  • The polyhedrin gene is not essential for the fundamental nucleocapsid assembly pathway.
  • AcMNPV nucleocapsid formation involves a distinct process of tube assembly, DNA incorporation, and segmentation.
  • This provides insights into the structural organization of baculoviral particles.

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