[Nucleotide sequence of gltB gene encoding the large subunit of Rhodobacter sphaeroides glutamate synthase]

The complete nucleotide sequence of a 5.4-kb chromosomal EcoRI-SalI fragment was determined, which contains the structural gene (gltB) for the large subunit of Rhodobacter sphaeroides glutamate synthase, as well as the 5'- and 3'- flanking regions. A open reading frame of 4636 base pairs was identified as R. sphaeroides gltB gene. The MW of the large subunit, as deduced from the nucleotide sequence, was estimated as 164kD. Comparision of the nucleotide sequences revealed a high similarity among gltB genes of R. sphaeroides, Azospirillum brasilense and Escherichia coli. The deduced amino acid sequence of R. sphaeroides GltB showed a high similarity with that of A. brasilense GltB.