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Effect induced by interleukin-1 on the behaviour of B16F10 melanoma cells
A G De Galdeano1, D Boyano, L Cañavate
1Department of Cell Biology and Morphological Sciences, School of Medicine and Dentistry, University of the Basque Country, Leioa 48940, Vizcaya, Spain.
We have previously demonstrated that in vitro treatment with interleukins (IL-2 and IL-6) enhances metastasis in B16 murine melanoma. IL-1 has been reported to be involved in the metastatic activity of human melanoma; so in the present study we have investigated if IL-1 could also modulate the behaviour of this experimental tumor. Data showed that low doses of IL-1 induced cell proliferation and several modifications in the expression of MHC antigens, fibronectin and laminin, however these changes did not lead to a higher metastatic efficiency. In addition it was shown that B16F10 cells express IL-1 mRNA. It can be concluded that IL-1 may be involved in the biology of B16F10 cells although its role in metastasis needs further investigation.
We have previously demonstrated that in vitro treatment with interleukins (IL-2 and IL-6) enhances metastasis in B16 murine melanoma. IL-1 has been reported to be involved in the metastatic activity of human melanoma; so in the present study we have investigated if IL-1 could also modulate the behaviour of this experimental tumor. Data showed that low doses of IL-1 induced cell proliferation and several modifications in the expression of MHC antigens, fibronectin and laminin, however these changes did not lead to a higher metastatic efficiency. In addition it was shown that B16F10 cells express IL-1 mRNA. It can be concluded that IL-1 may be involved in the biology of B16F10 cells although its role in metastasis needs further investigation.