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[Using autoclaved spongiosa]
E H Kuner1, W Schlickewei, M Huber-Lang
1Abteilung Unfallchirurgie, Chirurgische Universitätsklinik Freiburg.
Der Unfallchirurg
|December 29, 1998
Summary
Autoclaving allogenic cancellous bone offers a sterile, biocompatible, and osteoconductive bone graft alternative. While biologically inferior to other methods, it proves suitable for select indications, requiring further research into ultrastructural changes.
Area of Science:
- Biomaterials Science
- Orthopedic Surgery
- Tissue Engineering
Context:
- Transmission of Human Immunodeficiency Virus (HIV) via allogenic bone grafts necessitates reconsidering preservation methods.
- Existing cryopreservation guidelines are resource-intensive (labor, time, cost).
- Autoclaving presents a potential alternative for processing allogenic cancellous bone.
Purpose:
- To evaluate autoclaving as a method for processing allogenic cancellous bone for grafting.
- To assess the characteristics and suitability of autoclaved cancellous bone for clinical use.
- To identify areas for further investigation regarding the effects of autoclaving on bone tissue.
Summary:
- Allogenic cancellous bone, harvested from femoral heads, is cleaned and autoclaved at 134°C and 2.5 atm.
- This process removes organic matter, rendering the bone sterile, biocompatible, and osteoconductive, preserving its anorganic architecture.
- While biologically less effective than autogenic or cryopreserved bone, autoclaved grafts are suitable for specific indications.
Impact:
- Autoclaved cancellous bone provides a readily available, sterile bone graft material.
- It serves as a viable substitute in selected orthopedic procedures, reducing reliance on more complex preservation methods.
- Further research is needed to fully understand ultrastructural changes and identify specific proteins affected by autoclaving.