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Effect of Fenoterol on PAF-induced lung edema in isolated and perfused rabbit lungs
L Pesce1, S Tristano, E Friedman
1Unidad de Fisiologia Respiratoria, Instituto de Medicina Experimental, Facultad de Medicina, Escuela Luis Razetti, Universidad Central de Venezuela, Caracas.
Abstract:
We have studied the effects of fenoterol on PAF-induced response in pulmonary circulation. We used 28 isolated and perfused rabbit lungs preparations: eight control preparations (CP), four vehicles preparations (VP), eight PAF preparations (PP) with two doses of PAF, one called low dose (LD = 0.5 microg/kg of weight) and the other high dose (HD = 1 microg/kg of weight) and eight Fenoterol preparations (FP) which we administered 0.05 mg of Fenoterol for 15 min, followed by a LD and HD of PAF. FP prevented elevation of pulmonary artery pressure (Ppa) as compared to PP, at LD of PAF: 12.615 (CI 95%: 8.57-20.885) versus 83.705 (CI 95%: 50.55-114.3) cm of water; and at HD of PAF: 19.38 (CI 95%: 11.235-28.94) versus 205.1 (CI 95%: 141.3-271) cm of water respectively. FP prevented the increase in fluid filtration rate (FFR) observed in PP at both doses of PAF LD: 0.765 (CI 95%: 0.07-3.385) versus 0.01 (CI 95%: -0.05-0.005) g/min; HD: 5.515 (CI 95%: 2.425-8.865) versus 0.03 (CI 95%: 0-0.33) g/min. Our results suggest that PAF has a vasoconstrictor effect that produces lung edema and this effect is inhibited by fenoterol.
Insights
Fenoterol effectively inhibited pulmonary hypertension and lung edema caused by platelet-activating factor (PAF). This study demonstrates fenoterol
Area of Science:
- Pulmonary Circulation Research
- Pharmacology
- Respiratory Physiology
Background:
- Platelet-activating factor (PAF) is implicated in pulmonary vasoconstriction and edema.
- Understanding the mechanisms of PAF-induced pulmonary responses is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the effects of fenoterol on platelet-activating factor (PAF)-induced pulmonary hypertension and edema.
- To determine if fenoterol can mitigate the adverse effects of PAF in isolated rabbit lungs.
Main Methods:
- Utilized 28 isolated and perfused rabbit lungs.
- Administered varying doses of PAF (low and high) to induce pulmonary responses.
- Treated experimental groups with fenoterol prior to PAF administration to assess its inhibitory effects.
Main Results:
- Fenoterol significantly prevented the elevation of pulmonary artery pressure (Ppa) induced by both low and high doses of PAF.
- Fenoterol administration markedly reduced the fluid filtration rate (FFR) associated with PAF exposure.
- PAF induced significant pulmonary vasoconstriction and increased lung edema, effects reversed by fenoterol.
Conclusions:
- Platelet-activating factor (PAF) exerts a potent vasoconstrictor effect on the pulmonary circulation, leading to lung edema.
- Fenoterol demonstrates significant efficacy in inhibiting PAF-induced pulmonary hypertension and edema in an isolated lung model.
- These findings suggest fenoterol as a potential therapeutic agent for conditions involving PAF-mediated pulmonary dysfunction.