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[Multidrug-resistant tuberculosis. 1. Drug susceptibility testing and its standardization]
1Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo, Japan.
Kekkaku : [Tuberculosis]
|December 29, 1998
Summary
Standardizing drug susceptibility testing for tuberculosis is crucial for treatment and research. A proposed proportion method using Ogawa egg medium offers a standardized approach for mycobacteriology laboratories.
Area of Science:
- Mycobacteriology
- Clinical Microbiology
- Infectious Diseases
Context:
- Drug susceptibility testing (DST) for mycobacteria lacks international standardization, hindering comparative research and treatment guidance.
- Accurate DST is essential for managing tuberculosis (TB) and monitoring drug resistance.
- Previous efforts by the Japanese Society of Tuberculosis aimed to address standardization challenges.
Purpose:
- To introduce and describe a proposed standardized drug susceptibility testing procedure for mycobacteria.
- To establish critical drug concentrations and resistance thresholds for key anti-TB agents.
- To facilitate international comparability of DST results.
Summary:
- A new proportion method for DST using Ogawa egg medium is proposed, aligning with WHO recommendations.
- Resistance is defined as >1% of colonies growing on critical drug concentrations (isoniazid, rifampicin, streptomycin, ethambutol).
- This method aims to standardize DST in Japan, with future discussions on agar- and liquid-based media.
Impact:
- Enables standardized evaluation of chemotherapeutic trials for tuberculosis.
- Supports epidemiological surveys on the prevalence of drug-resistant tuberculosis strains.
- Provides a reliable basis for guiding tuberculosis patient treatment decisions.