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Colloidal Au-enhanced surface plasmon resonance immunosensing

L A Lyon1, M D Musick, M J Natan

  • 1Department of Chemistry, Pennsylvania State University, University Park 16802, USA.

Analytical Chemistry
|December 30, 1998
PubMed
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Colloidal gold nanoparticles significantly enhance surface plasmon resonance (SPR) biosensing sensitivity. This gold nanoparticle enhancement enables picomolar detection of analytes, improving SPR biosensing for protein interactions.

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Surface Plasmon Resonance (SPR) is a label-free optical technique widely used for detecting biomolecular interactions.
  • Enhancing SPR sensitivity is crucial for detecting low-concentration analytes and subtle biological events.
  • Colloidal gold nanoparticles offer unique optical properties that can be leveraged for signal amplification.

Purpose of the Study:

  • To investigate the use of colloidal gold nanoparticles for enhancing SPR biosensing.
  • To evaluate the impact of gold nanoparticle immobilization on SPR signal characteristics.
  • To demonstrate the application of this enhanced SPR technique for sensitive detection of protein-protein interactions and immunoassays.

Main Methods:

  • Immobilization of approximately 11-nm-diameter colloidal gold nanoparticles onto an evaporated gold film.

Related Experiment Videos

  • Utilizing a binding pair of gold film-immobilized antibody and antigen-colloidal gold conjugate.
  • Development of a particle-enhanced sandwich immunoassay by conjugating gold nanoparticles to a secondary antibody.

    • Main Results:

    • Colloidal gold immobilization caused a significant shift in plasmon angle, resonance broadening, and increased minimum reflectance.
    • Achieved a 25-fold signal amplification compared to free antibodies, enabling picomolar detection of human immunoglobulin G.
    • Demonstrated a quasi-linear relationship between particle coverage and plasmon angle shift, correlating signal to analyte concentration.

    Conclusions:

    • Colloidal gold enhancement significantly increases the sensitivity and generality of SPR biosensing.
    • The developed particle-enhanced immunoassay offers tremendous signal amplification for sensitive biomolecular detection.
    • This approach holds potential for highly sensitive, label-free detection of various analytes in biological samples.