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Related Experiment Videos

Stable cationic capillary coating with successive multiple ionic polymer layers for capillary electrophoresis

H Katayama1, Y Ishihama, N Asakawa

  • 1Department of Analytical Chemistry, Analytical Research Laboratories, Eisai Co., Ltd., Ibaraki, Japan. h1-katayama@eisai.co.jp

Analytical Chemistry
|December 30, 1998
PubMed
Summary

A novel successive multiple ionic-polymer (SMIL) coating enhances capillary stability for reproducible protein and drug analyses. This improved method ensures reliable results across numerous runs and harsh chemical conditions.

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrophoresis (CE) requires stable capillary coatings for reproducible analyses.
  • Conventional coatings often lack sufficient stability against harsh chemical environments and repeated use.
  • Polybrene (PB)-modified capillaries are useful for protein analysis but can suffer from stability issues.

Purpose of the Study:

  • To develop a novel coating procedure for enhanced capillary stability.
  • To improve the reproducibility and robustness of Polybrene (PB)-modified capillaries.
  • To demonstrate the applicability of the new coating for protein and cationic drug analyses.

Main Methods:

  • Developed a successive multiple ionic-polymer (SMIL) coating technique.
  • Involved sequential layering of cationic and anionic polymers onto the capillary inner wall.

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  • Tested the stability of the SMIL-coated Polybrene (PB) capillary against chemical agents and repeated analyses.

    • Main Results:

    • The SMIL-coated PB capillary demonstrated significantly improved stability, enduring over 600 replicate analyses.
    • The coating exhibited excellent stability in 1 M NaOH and 0.1 M HCl.
    • Achieved relative standard deviations below 1% for run-to-run, day-to-day, and capillary-to-capillary variations, indicating high reproducibility.
    • Successfully applied the coated capillary for protein analysis near the protein's isoelectric point (pI) and for adsorptive samples like plasma.
    • Enabled efficient capillary electrophoresis/mass spectrometry (CE/MS) analysis of cationic drugs.

    Conclusions:

    • The SMIL coating procedure provides a highly stable and reproducible modification for capillaries.
    • This method enhances the performance of Polybrene (PB)-modified capillaries for challenging analytical applications.
    • The developed technique is suitable for sensitive protein and cationic drug analyses using CE/MS.