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Microsatellite variation in Scandinavian Cervidae using primers derived from Bovidae
1Department of Morphology, Genetics and Aquatic Biology, Norwegian College of Veterinary Medicine, Oslo, Norway. knut.roed@vetinst.no
Hereditas
|December 30, 1998
Summary
Researchers explored using Bovidae microsatellite primers for Cervidae DNA analysis. Ovine/caprine primers showed higher success rates, yielding valuable polymorphic markers for moose, red deer, reindeer, and roe deer populations.
Area of Science:
- Genetics
- Zoology
- Molecular Biology
Background:
- Microsatellite markers are crucial for wildlife population genetics.
- Primers developed for one family may be applicable to related families.
Purpose of the Study:
- To assess the cross-family utility of Bovidae-derived microsatellite primers in Cervidae species.
- To identify polymorphic microsatellite markers for cervid population studies.
Main Methods:
- Tested 75 microsatellite primer sets (bovine, ovine, caprine) on DNA from four Scandinavian cervids: moose, red deer, reindeer, and roe deer.
- Analyzed PCR amplification success and polymorphism rates.
Main Results:
- Ovine/caprine primers amplified targets in ~50% of cervids, while bovine primers succeeded in only 16%.
- Approximately 50% of successful amplifications revealed polymorphisms.
- Identified 10-21 polymorphic markers per cervid species.
- Reindeer exhibited higher polymorphism, allele numbers, and heterozygosity.
Conclusions:
- Bovidae microsatellite primers, particularly ovine/caprine, are a viable tool for cervid genetic research.
- This approach efficiently identifies polymorphic markers for population genetics in moose, red deer, reindeer, and roe deer.
- Reindeer possess greater genetic diversity detectable by these markers.