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Macrophage migration inhibitory factor expression in human renal allograft rejection
1Department of Nephrology, Monash Medical Centre, Clayton, Victoria, Australia. Huilan@its-mmcc1.cc.monash.edu.au
Transplantation
|December 30, 1998
Summary
Macrophage migration inhibitory factor (MIF) is upregulated in kidney allograft rejection, correlating with immune cell infiltration and disease severity. This suggests MIF plays a key role in transplant rejection.
Area of Science:
- Immunology
- Nephrology
- Transplantation
Background:
- Macrophage migration inhibitory factor (MIF) is crucial in immune-mediated diseases.
- MIF's role in allograft rejection was previously unknown, despite its association with delayed-type hypersensitivity.
Purpose of the Study:
- To investigate the role and expression of MIF in human renal allograft rejection.
Main Methods:
- Assessed MIF expression using in situ hybridization and immunohistochemistry.
- Analyzed 62 human renal allograft rejection biopsies and normal kidney samples.
Main Results:
- MIF is constitutively expressed in normal kidneys, primarily in tubular and vascular cells.
- Renal allograft rejection showed marked upregulation of MIF in intrinsic kidney cells and infiltrating macrophages and T cells.
- Increased MIF expression significantly correlated with immune cell infiltration, rejection severity, and loss of renal function.
Conclusions:
- This study is the first to show elevated local MIF expression during renal allograft rejection.
- Up-regulated MIF, immune cell infiltration, and rejection severity suggest MIF is a significant mediator in allograft rejection.