Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A two-step digestion process and LAP-I cold preservation solution for human islet isolation

Y Mullen1, S Arita, T Kenmochi

  • 1Department of Surgery, UCLA School of Medicine, USA. ymullen@ucla.edu

Annals of Transplantation
|January 1, 1997
PubMed
Summary

Researchers developed a simplified pancreatic islet isolation method using a new preservation solution and a two-step digestion process. This cost-effective technique aims to improve outcomes for type 1 diabetes patients undergoing transplantation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nanoliposomal irinotecan with fluorouracil and folinic acid in patients with unresectable or recurrent pancreatic cancer: a multicenter observational study (NAPOLEON-2).

ESMO gastrointestinal oncology·2026
Same author

Tobacco smoking and tooth loss in adults and elderly in Brazil: a populational-based cross-sectional study.

Community dental health·2024
Same author

Effectiveness of Preceding Solo Kidney Transplantation for Type 1 Diabetes With End-Stage Renal Failure.

Transplantation proceedings·2018
Same author

Peptidylarginine deiminase is involved in maintaining the cornified oral mucosa of rats.

Journal of periodontal research·2018
Same author

Heat stress prevents the decrease in succinate dehydrogenase activity in the extensor digitorum longus of streptozotocin-induced diabetic rats.

Physiological research·2017
Same author

Gene Expression Profiling of Peripheral Blood From Kidney Transplant Recipients for the Early Detection of Digestive System Cancer.

Transplantation proceedings·2017

Area of Science:

  • Transplantation immunology
  • Endocrinology
  • Surgical innovation

Background:

  • Pancreatic islet transplantation is a promising treatment for type 1 diabetes, but long-term success is limited.
  • Current islet isolation methods require high donor pancreas quality and extensive expertise, hindering broader application.
  • Improving islet isolation efficiency and reducing costs are critical for advancing transplantation therapies.

Purpose of the Study:

  • To develop an improved, cost-effective pancreatic islet isolation method.
  • To simplify the procedure, requiring less specialized personnel and donor pancreas quality.
  • To enhance the yield of healthy islets for transplantation.

Main Methods:

  • Developed a novel preservation solution (LAP-I).

Related Experiment Videos

  • Implemented a two-step pancreas digestion: initial warm collagenase digestion followed by cold mechanical digestion.
  • Utilized a clear plastic digestion chamber for process visualization and fragment removal.

    • Main Results:

    • The modified procedure is simple, straightforward, and cost-effective.
    • Requires less manpower compared to conventional methods.
    • Aims to maximize the number of healthy islets obtained from donor pancreases.

    Conclusions:

    • The novel islet isolation technique offers significant advantages over existing methods.
    • This approach has the potential to increase the accessibility and success rate of pancreatic islet transplantation.
    • Further research and clinical application are warranted to validate these findings for type 1 diabetes treatment.