Accelerated study on lysozyme deposition on poly(HEMA) contact lenses

Area of Science:

• Biomaterials Science
• Ophthalmic Research
• Protein Chemistry

Background:

• Protein deposition, particularly lysozyme, is a common issue with contact lens wear, potentially leading to discomfort and reduced lens performance.
• Poly(2-hydroxyethyl methacrylate) (poly(HEMA)) is a widely used material for soft contact lenses.
• Effective cleaning solutions are crucial for maintaining contact lens hygiene and wearer comfort.

Purpose of the Study:

• To develop and utilize a technique for accelerated lysozyme deposition on poly(HEMA) contact lenses.
• To evaluate the efficacy of bendazac lysine solution in reducing lysozyme deposition.
• To investigate the influence of temperature and cleaning methods on lysozyme accumulation.

Main Methods:

• Lysozyme deposition on poly(HEMA) contact lenses was accelerated and quantified.
• Experiments were conducted at temperatures ranging from 25°C to 90°C to assess temperature effects.
• The effectiveness of bendazac lysine solution was tested under various cleaning conditions, including rubbing and agitation.

Main Results:

• Lysozyme deposition increased significantly with temperature, rising from 0.27 µg/lens at 25°C to 31 µg/lens at 90°C.
• Lysozyme deposition kinetics showed a rapid increase within the first 2 hours, followed by a plateau.
• Bendazac lysine reduced deposited lysozyme by over 40% when lenses were washed without rubbing, but its effect was negligible with finger rubbing.

Conclusions:

• Temperature is a critical factor influencing lysozyme deposition rates on poly(HEMA) contact lenses.
• Bendazac lysine solution demonstrates effectiveness in reducing lysozyme buildup, particularly when combined with agitation during storage.
• Contact lens cleaning methods significantly impact the efficacy of cleaning solutions; manual rubbing diminishes the benefit of bendazac lysine.