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Related Experiment Videos

Dendritic and postsynaptic protein synthetic machinery

A Gardiol1, C Racca, A Triller

  • 1Laboratoire de Biologie Cellulaire de la Synapse Normale et Pathologique, Institut National de la Santé et de la Recherche Médicale U497, Ecole Normale Supérieure, 75005 Paris, France.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|December 31, 1998
PubMed
Summary

Local protein synthesis in dendrites supports synaptic plasticity. This study identifies ribosomes and endoplasmic reticulum components within dendrites, enabling local protein production for synaptic function.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Emerging evidence suggests local protein synthesis in dendrites is crucial for synaptic plasticity.
  • Previous studies indicated dendrites possess translational and glycosylation capabilities.
  • The precise cellular structures involved in non-perinuclear translation remained largely unidentified.

Purpose of the Study:

  • To identify cellular structures responsible for dendritic and postsynaptic protein synthesis.
  • To investigate the presence and localization of protein synthesis machinery within neuronal dendrites.
  • To determine if synapses are equipped for local synthesis and modification of transmembrane proteins.

Main Methods:

  • Morphological and immunocytochemical analysis of rat spinal cord neurons.

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  • Utilized light and electron microscopy.
  • Employed antibodies against eukaryotic protein synthetic machinery (ribosomes, eIF-2) and organelle markers (BiP, rab1, CTR433, TGN38).

    • Main Results:

    • Protein synthesis macrocomplexes (ribosomes, eIF-2) and endomembranous systems (ER, Golgi) were found within dendrites.
    • Subsynaptic organelles, distinct in size and shape, were observed near postsynaptic densities.
    • These organelles showed immunoreactivity for ribosomal proteins, eIF-2, and markers of ER, intermediate compartment, and Golgi.

    Conclusions:

    • Synapses possess the necessary components for local synthesis of proteins.
    • Dendrites are equipped for cotranslational and posttranslational modifications, including glycosylation.
    • These findings support a model where local protein synthesis at synapses underlies synaptic plasticity.