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Sequence analysis of small cryptic plasmids isolated from Selenomonas ruminantium S20
M Nakamura1, T Nagamine, K Ogata
1Rumen Microbiology Research Team, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba Tsukuba, Ibaraki 305-0854, Japan.
Current Microbiology
|December 31, 1998
Summary
Two small cryptic plasmids, pONE429 and pONE430, were isolated from Selenomonas ruminantium. These plasmids exhibit conserved regions potentially involved in host recognition and rolling circle replication (RCR).
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Rumen bacteria, such as Selenomonas ruminantium, harbor cryptic plasmids that can influence host cell characteristics.
- Understanding plasmid biology is crucial for genetic manipulation and studying bacterial adaptation.
Purpose of the Study:
- To characterize two novel cryptic plasmids, pONE429 and pONE430, isolated from Selenomonas ruminantium S20.
- To investigate the replication mechanism and potential host interaction regions of these plasmids.
Main Methods:
- Plasmid DNA isolation from Selenomonas ruminantium S20.
- Complete nucleotide sequencing of plasmids pONE429 and pONE430.
- Bioinformatic analysis to identify open reading frames (ORFs) and conserved regions.
Main Results:
- Plasmid pONE429 (2100 bp) and pONE430 (1527 bp) were fully sequenced, each containing a single ORF.
- The replication protein (Rep protein) of pONE430 showed similarity to Rep proteins from other bacterial species.
- Conserved nucleotide sequences upstream of the Rep protein suggest a double-strand origin (DSO) for rolling circle replication (RCR).
- A conserved region (<450 bp) was identified across multiple S. ruminantium plasmids, potentially mediating host recognition.
Conclusions:
- The identified plasmids pONE429 and pONE430 possess features consistent with rolling circle replication (RCR).
- The conserved nucleotide sequences indicate a potential mechanism for replication and host-specific interaction within S. ruminantium strains.