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Column liquid chromatography determination of vitamins A and E in powdered milk and local flour: a validation
1Laboratoire de Chimie Analytique, Faculté de Pharmacie, Montpellier, France.
Abstract:
A high-performance liquid chromatography method was developed for the simultaneous routine determination of vitamins A and E in powdered milk and flour made from local plants and purchased from open markets in the Ivory Coast. The method involves saponification followed by extraction with a mixture of organic solvents. The vitamins were resolved by reversed-phase HPLC and detection at a single wavelength. The main tests of method validation were applied to the procedure. The results show the reliability of the analytical method for the intended application.
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