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A fast and inexpensive method for N-terminal fluorescein-labeling of peptides
P J Weber1, J E Bader, G Folkers
1Department of Pharmacy, Swiss Federal Institute of Technology (ETH), Zürich, Switzerland.
Bioorganic & Medicinal Chemistry Letters
|January 1, 1999
Abstract:
A new method has been developed to synthesize fluorescein labeled peptides, compounds of increasing importance in bioorganic chemistry, cell biology, pharmacology, drug targeting and medicinal chemistry. We show, that 4(5)-carboxyfluorescein is much more efficient than the hitherto predominantly utilized reagents 4(5)-carboxyfluorescein-N-succinimidylester and 4(5)-fluoresceinisothiocyanate.