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Substrate specificity of human prolyl-4-hydroxylase
1Department of Chemistry, Cornell University, Ithaca, New York 14853, USA.
Bioorganic & Medicinal Chemistry Letters
|January 1, 1999
Abstract:
Proline analogs (3-F, 3-Cl, 3-Br, 3,3-cyclopropyl, 3,3-methylene, 3-Me, and 4-Me) were synthesized, incorporated into CbzGlyPheXGlyOEt, and tested as substrate analogues/mechanistic probes for the human prolyl-4-hydroxylase catalyzed hydroxylation reaction. With the exception of the 3-fluoroproline containing peptide, none of these peptides were substrates for the enzyme.