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alpha-Mannosidase activity from antibody raised against a glucal antigen
J Yu1
1Division of Applied Science, Korea Institute of Science & Technology, Seoul, Korea.
Bioorganic & Medicinal Chemistry Letters
|January 1, 1999
Abstract:
Sixty cell lines of monoclonal antibody were raised against a glucal hapten 1. Among them, Ab 405.4 showed alpha-mannosidase activity as kcat = 0.19/day (kcat/kuncat = 110,000). The chemical modification study and pH profile study of this antibody indicated that carboxyl group(s) in the antigen binding site involved in catalytic mechanism.