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Synthesis of fluorescent substrates for protein tyrosine phosphatase assays
T Watanabe1, M Imoto, H Taniguchi
1Institute of Microbial Chemistry, Tokyo, Japan.
Bioorganic & Medicinal Chemistry Letters
|January 1, 1999
Abstract:
Two fluorescent substrates for protein tyrosine phosphatase (PTPase) reaction were prepared by conjugation of commercially available O-phosphotyrosine and dansyl chlorides. They were hydrolyzed by CD45 tyrosine phosphatase, and proved to be useful for PTPase assay.