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A stable reagent system for screening and identifying red blood cell irregular antibodies: application to commercial
L Million1, C Pellerin, M Marchand-Arvier
1Laboratoire d'Hématologie-Physiologie, Faculté de Pharmacie, Nancy, France.
Objective:
Development of a new solid-phase system for screening and identifying irregular red cell antibodies.
Materials And Methods:
Red blood cell membranes were prepared by a semi-automated procedure in which the hemolysate solution was passed through a hollow-fiber system. The membranes were fixed to the solid phase (microtiter plates) by centrifugation and incubated with 8% fat-free milk. Antibodies added to the microtiter plate were detected by anti-human antibodies adsorbed onto yellow latex particles.
Results:
The system had good sensitivity (titer <1); 97% of anti-D samples were detected. The detection system was stable for 6 months at 4 degrees C.
Conclusion:
This stable-antigen solid-phase system readily detects and identifies red cell antibodies that are important in transfusion.